![]() PARTICIPANTS: Co-investigators are University of California, Davis veterinary faculty Joan Dean Rowe and Bruno Chomel. Peer and industry publications planned upon completion of analysis. Extension Goat Day) and veterinary practitioners (AASRP/ADGA/AABP) presentations. Future dissemination upon completion of analysis will be to goat producer groups (2012 U.C. Kreutzberg previously presented preliminary herd Coxiella data at 2009 US Army CHPPM Force Health Protection Conference and during the report period at 2010 U.C. DISSEMINATION: Preliminary data were shared locally with participating producers and their personnel. Reproductive outcomes and CB shedding data for all enrolled animals/herds are now completed and data analyses are in progress. Reproductive outcomes (subsequent kidding, abortion, conception, culling) data collection is complete and quantitative PCR analysis for lochia during the reporting period. Correlation between Coxiella Phase I and Phase II IFA tests and ELISA serologic tests are determined. Preliminary data analyzed for association between CAEV infection status and Coxiella infection was reported in prior progress report. Progress 01/01/10 to 12/31/10 Outputs OUTPUTS: OUTPUTS: Activities: Data collection completed serologic testing (CAEV and Coxiella burnetti (CB) and & PCR testing (CB)of vaginal (and and male samples) samples now complete for the study goat herds. No additional outcomes for this unfunded reporting period, pending final analysis of multi-year follow-up and completion of final report. Impacts Current reporting period: Preliminary outcomes were reported in last reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PARTICIPANTS: Nothing significant to report during this reporting period. DISSEMINATION: CAEV & Coxiella epidemiologic findings form this project were included presented in infectious disease control and abortion seminars at 2 veterinary continuing education conferences and 1 producer conference during the reporting period. Funding for the project ended during the previous reporting period project outputs will be updated in final report during next reporting period. Progress 01/01/11 to 12/31/11 Outputs OUTPUTS: Activities: During the reporting period, final assays were completed and data completed. Multiway tables and chi-square analysis will be used for cross-classified data with categorical variables. Analysis of variance will be used to examine the associations and interactions among CAEV infection status, intramammary bacterial infection status, mean lactation somatic cell count, 305 day milk yield, as well as butterfat and protein composition. Descriptive statistics will be used to assess the comparability of groupsīy breed, parity and prior mastitis status. Number of clinical cases will be recorded and owner will aseptically collect and freeze a milk sample prior to treatment for later culture. Milk cultures will be performed using washed cow blood agar and mycoplasma media using standard microbial methods for milk.10 Goats will be categorized for bacterial IMI as not infected, intermittently infected or persistently infected and, if sufficient numbers, grouped by agent. ![]() ![]() Milk samples will be aseptically collected using standard procedures. Additional milk cultures will be taken on does with clinical mastitis or with SCC exceeding 800,000/ml at any monthly test. Goats will be tested for CAEV and milk cultured for bacteria for intramammary infection (IMI) at enrollment (freshening), mid-lactation (150 days in milk) and the end of lactation. Goats with clinically enlarged joints (as determined by the investigator) will be excluded from the study. ![]() DHIA records will be reviewed on a monthly basis for change in SCC and to record 305-day milk yieldĪnd composition data. Breed, parity, kidding group (month) and prior mastitis history will be recorded. Serum ELISA test for CAEV antibody and PCR of peripheral blood mononuclear cells (PBMC) for CAEV pro-viral DNA (as previously described) will be used to determine infection status at enrollment and to categorize goats as uninfected, infected during the study period,or infected throughout the study period. ![]() We expect an 8-month enrollment period (starting at beginning of fall kidding) and 10 month follow-up per animal. Animals will be pre-screened for enrollment as they approach kidding due date. Project Methods CAEV-infected does (n=140), as determined by positive serum CAEV-ELISA and PBMC CAEV-PCR,without signs of joint enlargement (sign of clinical CAEV infection) and CAEV-uninfected does (n=160), as determined by negative CAEV-ELISA and PBMC CAEV-PCR tests) will be will be enrolled at time of freshening. ![]()
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